ISO 20776 PDF

ISO 20776 PDF

ISO. First edition. Clinical laboratory testing and in vitro diagnostic test systems — Susceptibility testing of infectious agents and evaluation. ISO () Clinical laboratory testing and in vitro diagnostic test systems – Susceptibility testing of infectious agents and evaluation of performance of. Buy ISO , Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices, Part 1, at CLSI.

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However, preparation of ITC trays for antimicrobial susceptibility testing AST microdilution tests is associated with a significant lack of reproducibility, particularly after freezing and shipping, independent of which dilution method was followed.

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First indications of improved capability and capacity of public health microbiology laboratories in the European Union http: A multicenter study was conducted to assess the accuracy of the ISO standard and the serial 2-fold dilution procedures for ido susceptibility testing. Precision of itraconazole dilution series by procedure and laboratory a.

The method was validated by performing three calibration curves on consecutive days. International Organization for Standards, Geneva, Switzerland.

ITC is a weakly basic drug pKa ca. Sitemap Search close sitemap. Susceptibility testing of yeasts Simultaneous quantification of voriconazole and posaconazole in human plasma by high-performance liquid chromatography with ultra-violet detection.


HPLC is a sensitive and reproducible method for determining levels of azole components in a great variety of matrices, as has been confirmed in previous reports 12510 – 121519 – 21 Reference method for broth dilution antifungal susceptibility testing of filamentous fungi, 2nd ed. In summary, our data suggest that microdilution trays for hydrophilic drugs such as FLC can be accurately prepared following the ISO recommendations or the serial 2-fold dilution schemes.

Relevant external documents

Sensitive determination of itraconazole and its active metabolite in human plasma by column-switching high-performance liquid chromatography with ultraviolet detection. Sio were done in duplicate on separate days at each center and then shipped frozen by special delivery to Spain for further chromatographic concentration determinations. Fifty microliters of the resultant mix was injected directly into the HPLC system.

Evaluation of performance of antimicrobial susceptibility test devices.

An optimized analytical method of fluconazole in human plasma by high-performance liquid chromatography with ultraviolet detection and its application to a bioequivalence study. Support Center Support Center. Precision of fluconazole dilution series by dilution procedure and laboratory a. Microtiter plates were prepared according to the ISO standard and by serial dilution procedures recommended for preparation of trays of both hydrophilic and hydrophobic drugs 9 Meetings, Minutes and Reports Previous versions of documents AST of mycobacteria Susceptibility testing of pathogenic fungi with itraconazole: These documents are available for purchase from ISO or national standards organisations.


Development and validation of a high-performance liquid chromatographic assay for the determination of fluconazole in human whole blood using solid phase extraction.

Development and validation of an efficient HPLC method for quantification of voriconazole in plasma and microdialysate reflecting an important target site. National Center for Biotechnology InformationU.

EUCAST: External documents

Bioanalytical method validation-a revisit with a decade of progress. All statistical analyses were done with the statistical package PAWS statistics Prevalidation statistical design to assess analytical methods.

ECDC documents of relevance izo antimicrobial susceptibility testing. Clinical breakpoints for antimycobacterial agents Thus, ITC data obtained from trays prepared by the Inn Austria participant were excluded from the analysis since tray wrappings showed evidence of defrosting and spillage; also, concentrations of dilutions prepared in Denmark were unacceptably different from the target concentrations.